Cullin 3 antibody (AA 650-700)

Catalog No. ABIN7453602

This anti-Cullin 3 antibody is a Rabbit Polyclonal antibody detecting Cullin 3 in WB, IP and IHC (fp). Suitable for Human and Mouse.

Quick Overview for Cullin 3 antibody (AA 650-700) (ABIN7453602)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Cullin 3 Antibody

Binding Specificity: AA 650-700

Purpose: Rabbit anti-Cul3 Antibody, Affinity Purified

Predicted Reactivity: Rat,X. laevis,X. tropicalis

Purification: Affinity Purified

Immunogen: between AA 650 and 700

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: Cul3

Background: Background: Cul3 is a member of the cullin group of proteins and functions as a scaffold to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. Cul3 and its associated ubiquitin ligase activity are essential for mitotic division and required for correct alignment of chromosomes and the completion of cytokinesis. Aurora B is a substrate of Cul3-based E3 ubiquitin ligase activity.

Gene ID: 8452

NCBI Accession: NP_003581

UniProt: Q13618

Pathways: M Phase