Cyclin T1 antibody (AA 425-475)

Catalog No. ABIN7453624

This Rabbit Polyclonal antibody specifically detects Cyclin T1 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Cyclin T1 antibody (AA 425-475) (ABIN7453624)

Target: Cyclin T1 (CCNT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin T1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Cyclin T1 Antibody

Binding Specificity: AA 425-475

Purpose: Rabbit anti-Cyclin T1 Antibody, Affinity Purified

Predicted Reactivity: Bovine,Horse,Chimpanzee

Purification: Affinity Purified

Immunogen: between AA 425 and 475

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Cyclin T1

Target: Cyclin T1 (CCNT1)

Alternative Name: Cyclin T1

Background: Background: Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA) [taken from the Universal Protein Resource (UniProt) O60563].

Gene ID: 904

NCBI Accession: NP_001231

UniProt: O60563