MED18 antibody (C-Term)

Catalog No. ABIN7453729

This Rabbit Polyclonal antibody specifically detects MED18 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for MED18 antibody (C-Term) (ABIN7453729)

Target: MED18 (Mediator Complex Subunit 18 (MED18))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED18 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MED18 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-MED18 Antibody, Affinity Purified

Predicted Reactivity: Zebrafish,Bovine

Purification: Affinity Purified

Immunogen: between AA 150 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MED18

Target: MED18 (Mediator Complex Subunit 18 (MED18))

Alternative Name: MED18

Background: Background: MED18 is a component of the multisubunit mediator complex that functions as a bridge between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. MED18 forms a subcomplex with the MED8 and MED20 subunits and plays an important role in initiation complex formation.

Gene ID: 54797

NCBI Accession: NP_060108

UniProt: Q9BUE0

Pathways: Regulation of Lipid Metabolism by PPARalpha