NFYA antibody (AA 333-347)

Catalog No. ABIN7453741

This Rabbit Polyclonal antibody specifically detects NFYA in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for NFYA antibody (AA 333-347) (ABIN7453741)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-NFYA Antibody

Binding Specificity: AA 333-347

Purpose: Rabbit anti-NF-YA Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 333 and 347

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NF-YA

Background: Background: Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.

Gene ID: 4800

NCBI Accession: NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha