RBM17 antibody (AA 351-401)

Catalog No. ABIN7453790

The Rabbit Polyclonal anti-RBM17 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect RBM17 in samples from Human.

Quick Overview for RBM17 antibody (AA 351-401) (ABIN7453790)

Target: RBM17 (RNA Binding Motif Protein 17 (RBM17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM17 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RBM17 Antibody

Binding Specificity: AA 351-401

Purpose: Rabbit anti-SPF45 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 351 and 401

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RBM17

Target: RBM17 (RNA Binding Motif Protein 17 (RBM17))

Alternative Name: SPF45

Background: Background: Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) Q96125].

Gene ID: 84991

NCBI Accession: NP_116294

UniProt: Q96I25