Symplekin antibody (AA 1224-1274)

Catalog No. ABIN7453798

This Rabbit Polyclonal antibody specifically detects Symplekin in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Symplekin antibody (AA 1224-1274) (ABIN7453798)

Target: Symplekin (SYMPK)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Symplekin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Symplekin Antibody

Binding Specificity: AA 1224-1274

Purpose: Rabbit anti-Symplekin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1224 and 1274

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Symplekin

Target: Symplekin (SYMPK)

Alternative Name: Symplekin

Background: Background: Symplekin is a component of tight junctions that also localizes to the nucleus and is a component of 3'-end processing machinery. As part of the mRNA 3'-end processing machinery symplekin is necessary for poly(A) tail elongation. Although histone mRNA 3'ends are not polyadenylated, symplekin has been shown to also play a role in 3'end maturation of histone mRNAs.

Gene ID: 8189

NCBI Accession: NP_004810

UniProt: Q92797