NHEJ1 antibody (C-Term)

Catalog No. ABIN7453811

The Rabbit Polyclonal anti-NHEJ1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect NHEJ1 in samples from Human.

Quick Overview for NHEJ1 antibody (C-Term) (ABIN7453811)

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHEJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-NHEJ1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-XLF Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 250 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Alternative Name: XLF

Background: Background: XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.

Gene ID: 79840

NCBI Accession: NP_079058

UniProt: Q9H9Q4

Pathways: DNA Damage Repair