Recombinant CD8 alpha antibody (AA 28-194)

Catalog No. ABIN7453877

The Rabbit Monoclonal anti-CD8 alpha antibody has been validated for WB, IP and IHC (fp). It is suitable to detect CD8 alpha in samples from Mouse.

Quick Overview for Recombinant CD8 alpha antibody (AA 28-194) (ABIN7453877)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR173J

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 28-194

Purpose: Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody [BLR173J]

Immunogen: residues 28-194 (ECD)

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 12 μL/1 mg lysate

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 500 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha

Background: Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731

Pathways: TCR Signaling