U2AF1 antibody (AA 140-190)

Catalog No. ABIN7453889

The Rabbit Polyclonal anti-U2AF1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect U2AF1 in samples from Human and Mouse.

Quick Overview for U2AF1 antibody (AA 140-190) (ABIN7453889)

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-U2AF1 Antibody

Binding Specificity: AA 140-190

Purpose: Rabbit anti-U2AF35 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 140 and 190

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for U2AF1

Target: U2AF1 (U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1))

Alternative Name: U2AF35

Background: Background: U2AF35 heterodimerizes with U2AF65 to form the U2 snRNP auxiliary factor which is part of the splicesome. U2AF35 is required for splicing of AG-dependent pre-mRNAs and functions in recognizing the conserved 3' splice site dinucleotide AG. U2AF35 contains an atypical RNA recognition motif (RRM), two C3H1-type zinc fingers, and a C-terminal arginine/serine (RS)-rich domain interrupted by glycines.

Gene ID: 7307

NCBI Accession: NP_006749

UniProt: Q01081