BAF53A antibody (AA 25-75)

Catalog No. ABIN7453898

The Rabbit Polyclonal anti-BAF53A antibody has been validated for WB and IHC (fp). It is suitable to detect BAF53A in samples from Human and Mouse.

Quick Overview for BAF53A antibody (AA 25-75) (ABIN7453898)

Target: BAF53A (Actin-Like 6A (BAF53A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAF53A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BAF53A Antibody

Binding Specificity: AA 25-75

Purpose: Rabbit anti-BAF53A Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 25 and 75

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BAF53A

Target: BAF53A (Actin-Like 6A (BAF53A))

Alternative Name: BAF53A

Background: Background: BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Gene ID: 86

NCBI Accession: NP_004292

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism