GPI antibody (AA 508-558)

Catalog No. ABIN7453918

The Rabbit Polyclonal anti-GPI antibody has been validated for WB, IP and IHC (fp). It is suitable to detect GPI in samples from Human.

Quick Overview for GPI antibody (AA 508-558) (ABIN7453918)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-GPI Antibody

Binding Specificity: AA 508-558

Purpose: Rabbit anti-GPI Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 508 and 558

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI

Background: Background: Glucose-6-phosphate isomerase (GPI) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. GPI functions in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment [taken from NCBI Entrez Gene (Gene ID: 2821)].

Gene ID: 2821

NCBI Accession: NP_000166

UniProt: P06744