TReP-132/TRERF1 (AA 1150-1200) antibody

Catalog No. ABIN7453934

The Rabbit Polyclonal anti-TReP-132/TRERF1 antibody (ABIN7453934) specifically detects TReP-132/TRERF1 in IP, IHC (fp) and WB. The antibody is reactive with Human samples.

Quick Overview for TReP-132/TRERF1 (AA 1150-1200) antibody (ABIN7453934)

Target: TReP-132/TRERF1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Product Details

Binding Specificity: AA 1150-1200

Purpose: Rabbit anti-TReP-132/TRERF1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1150 and 1200

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: TReP-132/TRERF1

Background: Background: The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.

Gene ID: 55809

NCBI Accession: NP_277037

UniProt: Q96PN7