Recombinant EPAS1 antibody (AA 400-450)

Catalog No. ABIN7454018

This anti-EPAS1 antibody is a Rabbit Monoclonal antibody detecting EPAS1 in WB, IHC, IP, ICC, IF/ICC and ChIP-seq. Suitable for Human.

Quick Overview for Recombinant EPAS1 antibody (AA 400-450) (ABIN7454018)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (fixed cells) (IF/ICC), ChIP DNA-Sequencing (ChIP-seq)

Clone: BL-95-1A2

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 400-450

Purpose: Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]

Immunogen: between AA 400 and 450

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: HIF2-alpha

Background: Background: The gene EPAS1 encodes for the transcription factor endothelial PAS domain-containing protein 1, and is induced as oxygen levels fall. Commonly called hypoxia inducible factor 2A (HIF2a), this protein is involved in the induction of oxygen regulated genes, binding to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. An important cancer biomarker, HIF2a may also play a role in Tibetan high-altitude adaptation (Am J Hum Genet. 2015 Jul 2, 97(1): 54-66.) and mutations in the EPAS1 gene are associated with erythrocytosis familial type 4 [taken from NCBI Entrez Gene (Gene ID: 2034) and UniProtKB (Entry: Q99814)].

Gene ID: 2034

NCBI Accession: NP_001421

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect