KDM4A antibody (AA 350-400)

Catalog No. ABIN7454377

The Rabbit Polyclonal anti-KDM4A antibody has been validated for IP and IHC (fp). It is suitable to detect KDM4A in samples from Human.

Quick Overview for KDM4A antibody (AA 350-400) (ABIN7454377)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4A antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KDM4A Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-JMJD2A Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-JMJD2A antibody A300-861A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KDM4A

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: JMJD2A

Background: Background: JMJD2A is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD2A is a trimethylation-specific demethylase and functions as a transcriptional repressor. JMJD2A interacts with pRb and class one histone deacetylases to mediate repression of E2F-regulated promoters.

Gene ID: 9682

NCBI Accession: NP_055478

UniProt: O75164

Pathways: Warburg Effect