CDT2/RAMP antibody (AA 375-425)

Catalog No. ABIN7454381

This anti-CDT2/RAMP antibody is a Rabbit Polyclonal antibody detecting CDT2/RAMP in IP and IHC (fp). Suitable for Human.

Quick Overview for CDT2/RAMP antibody (AA 375-425) (ABIN7454381)

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT2/RAMP antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CDT2/RAMP Antibody

Binding Specificity: AA 375-425

Purpose: Rabbit anti-DTL/CDT2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 375 and 425

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-DTL/CDT2 antibody A300-948A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Alternative Name: DTL/CDT2

Background: Background: DTL/CDT2 is a component of the CUL4A-DDB1 E3 ubiquitinating complex and plays a role in downregulating CDT1, an essential component of pre-replication complexes (Pre-RC) that ensures the genome is replicated only once per cycle. As part of the CUL4A-DDB1 E3 ligase complex DTL/CDT2 is also involved in the regulation of other proteins involved in the G2/M cell cycle checkpoint.

Gene ID: 51514

NCBI Accession: NP_057532

UniProt: Q9NZJ0