LUC7-Like 3 antibody (AA 1-50)

Catalog No. ABIN7454406

Product Details anti-LUC7-Like 3 Antibody

Target: LUC7-Like 3 (LUC7L3)

Binding Specificity: AA 1-50

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LUC7-Like 3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-CROP/Luc7A Antibody, Affinity Purified

Predicted Reactivity: Mouse,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. A301-498A has not performed satisfactorily when used for WB of CROP/Luc7A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CROP/Luc7A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for LUC7-Like 3

Target: LUC7-Like 3 (LUC7L3)

Alternative Name: CROP/Luc7A (LUC7L3 Products)

Background: Background: Cisplatin resistance-associated overexpressed protein (CROP) was originally cloned by differential display from cisplatin-resistant cells. It is an SR-related protein and the human homolog of the yeast protein, Luc7p. Luc7p is an essential protein involved in vegetative growth and 5'-splice recognition. CROP/Luc7a contains arginine- and glutamate-rich domains (RE domains) and arginine- and serine-rich domains (RS domains). It localizes to the nucleus and is distributed as speckles. CROP/Luc7a has been shown to interact with SF2/ASF, a protein involved in RNA splicing. Due to this association and the known function of its yeast homolog, CROP/Luc7a is proposed to play a role in RNA splicing.

Gene ID: 51747

NCBI Accession: NP_057508

UniProt: O95232

Pathways: Ribonucleoprotein Complex Subunit Organization