GRB10 antibody (AA 100-150)

Catalog No. ABIN7454420

This anti-GRB10 antibody is a Rabbit Polyclonal antibody detecting GRB10 in IP and IHC (fp). Suitable for Human.

Quick Overview for GRB10 antibody (AA 100-150) (ABIN7454420)

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB10 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-GRB10 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit anti-GRB10 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 100 and 150

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-GRB10 antibody A301-734A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GRB10

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Alternative Name: GRB10

Background: Background: Growth factor receptor-bound protein 10 (GRB10) is a member of a family of adaptor proteins that bind to a variety of growth factor receptors to regulate receptor signaling and mediate signals for a variety of processes such as metabolism, growth, apoptosis, and migration. As a dimer, via its BPS (between PH and SH2) and SH2 (src-homology 2) domains, GRB10 associates with the insulin receptor (IR) as well as the insulin-like growth factor receptor (IGFR). GRB10's association with the IR has been shown to have inhibitory effects on insulin-induced kinase signaling, however studies have also reported that GRB10 can also have positive effects on IR signaling. It is suggested that GRB10 can serve in cellular functions independent of an interaction with the IR.

Gene ID: 2887

NCBI Accession: NP_005302

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2