CASC3 antibody (AA 225-275)

Catalog No. ABIN7454451

This Rabbit Polyclonal antibody specifically detects CASC3 in IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CASC3 antibody (AA 225-275) (ABIN7454451)

Target: CASC3 (Cancer Susceptibility Candidate 3 (CASC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASC3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CASC3 Antibody

Binding Specificity: AA 225-275

Purpose: Rabbit anti-CASC3 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 225 and 275

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-CASC3 antibody A302-472A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CASC3

Target: CASC3 (Cancer Susceptibility Candidate 3 (CASC3))

Alternative Name: CASC3

Background: Background: Cancer susceptibility candidate gene 3 protein (CASC3) is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. CASC3 is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles [taken from NCBI Entrez Gene (GeneID: 22794)].

Gene ID: 22794

NCBI Accession: NP_031385

UniProt: O15234