ZEB2 antibody (AA 750-800)

Catalog No. ABIN7454452

The Rabbit Polyclonal anti-ZEB2 antibody has been validated for IP and IHC (fp). It is suitable to detect ZEB2 in samples from Human.

Quick Overview for ZEB2 antibody (AA 750-800) (ABIN7454452)

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-ZEB2 Antibody

Binding Specificity: AA 750-800

Purpose: Rabbit anti-ZEB2/SIP Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 750 and 800

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-ZEB2/SIP antibody A302-474A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: ZEB2/SIP

Background: Background: Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) O60315].

Gene ID: 9839

NCBI Accession: NP_055610

UniProt: O60315

Pathways: Tube Formation