EAF2 antibody (AA 210-260)

Catalog No. ABIN7454454

This Rabbit Polyclonal antibody specifically detects EAF2 in IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for EAF2 antibody (AA 210-260) (ABIN7454454)

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EAF2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EAF2 Antibody

Binding Specificity: AA 210-260

Purpose: Rabbit anti-EAF2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 210 and 260

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. A302-503A has not performed satisfactorily when used for WB of EAF2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of EAF2.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EAF2

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Alternative Name: EAF2

Background: Background: ELL-associated factor 2 (EAF2) acts as a transcriptional transactivator of TCEA1 elongation activity. It is also a transcriptional transactivator of ELL (eleven nineteen lysine-rich leukemia) 1 and ELL2 elongation activities. EAF2 has been shown to be a potent inducer of apoptosis in prostatic and non-prostatic cell lines and to inhibit prostate tumor growth in vivo [taken from the Universal Protein Resource (UniProt) Q96CJ1].

Gene ID: 55840

NCBI Accession: NP_060926

UniProt: Q96CJ1