BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 175-225) antibody

Catalog No. ABIN7454455

The Rabbit Polyclonal anti- antibody has been validated for IP and IHC (fp). It is suitable to detect in samples from Human.

Quick Overview for BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 175-225) antibody (ABIN7454455)

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details

Binding Specificity: AA 175-225

Purpose: Rabbit anti-MERIT40 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 175 and 225

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Alternative Name: MERIT40

Background: Background: MERIT40 (mediator of RAP80 interactions and targeting subunit of 40 kDa) is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. MERIT40 probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin [taken from the Universal Protein Resource (UniProt) Q9NWV8].

Gene ID: 29086

NCBI Accession: NP_054892

UniProt: Q9NWV8

Pathways: Positive Regulation of Response to DNA Damage Stimulus