Desmoplakin antibody (AA 1900-1950)

Catalog No. ABIN7454486

This anti-Desmoplakin antibody is a Rabbit Polyclonal antibody detecting Desmoplakin in IP and IHC (fp). Suitable for Human.

Quick Overview for Desmoplakin antibody (AA 1900-1950) (ABIN7454486)

Target: Desmoplakin (DSP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Desmoplakin Antibody

Binding Specificity: AA 1900-1950

Purpose: Rabbit anti-Desmoplakin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1900 and 1950

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: Desmoplakin

Background: Background: Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in DSP, the gene encoding desmoplakin, are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus [taken from NCBI Entrez Gene (Gene ID: 1832)].

Gene ID: 1832

NCBI Accession: NP_004406

UniProt: P15924

Pathways: Cell-Cell Junction Organization