WWOX antibody (AA 364-414)

Catalog No. ABIN7454501

This anti-WWOX antibody is a Rabbit Polyclonal antibody detecting WWOX in IP and IHC (fp). Suitable for Human.

Quick Overview for WWOX antibody (AA 364-414) (ABIN7454501)

Target: WWOX (WW Domain Containing Oxidoreductase (WWOX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWOX antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-WWOX Antibody

Binding Specificity: AA 364-414

Purpose: Rabbit anti-WWOX Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: Between AA 364 and 414

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. A304-079A has not performed satisfactorily when used for WB of WWOX in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of WWOX.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for WWOX

Target: WWOX (WW Domain Containing Oxidoreductase (WWOX))

Alternative Name: WWOX

Background: Background: WW domain-containing oxidoreductase (WWOX) is a putative oxidoreductase, acts as a tumor suppressor, plays a role in apoptosis, and is required for normal bone development (by similarity). WWOX may function synergistically with p53/TP53 to control genotoxic stress-induced cell death. It plays a role in TGFB1 signaling and TGFB1-mediated cell death and may also play a role in tumor necrosis factor (TNF)-mediated cell death. WWOX inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm [taken from the Universal Protein Resource (UniProt) Q9NZC7].

Gene ID: 51741

UniProt: Q9NZC7