BRD8 antibody (Isoform 2)

Catalog No. ABIN7454596

The Rabbit Polyclonal anti-BRD8 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect BRD8 in samples from Human.

Quick Overview for BRD8 antibody (Isoform 2) (ABIN7454596)

Target: BRD8 (Bromodomain Containing 8 (BRD8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BRD8 Antibody

Binding Specificity: AA 225-275, Isoform 2

Purpose: Rabbit anti-BRD8 Antibody, Affinity Purified

Predicted Reactivity: Rat,Dog,Horse,Rabbit,Guinea pig_10141,Pig,Panda,Monkey,Gorilla

Purification: Affinity Purified

Immunogen: between AA 225 and 275 of isoform 2

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 8 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BRD8

Target: BRD8 (Bromodomain Containing 8 (BRD8))

Alternative Name: BRD8

Background: Background: BRD8 (bromodomain-containing 8) has been identified as a thyroid hormone receptor coactivator as well as a GTPase activating protein (GAP) for Arf6 that regulates clathrin-dependent vesicle trafficking. BRD8 has also been identified as a gene upregulated during formation of the neointima layer of blood vessels and named smooth muscle activating protein 2 (SMAP2).

Gene ID: 10902

NCBI Accession: NP_631938

UniProt: Q9H0E9