Claspin antibody (AA 725-775)

Catalog No. ABIN7454603

This anti-Claspin antibody is a Rabbit Polyclonal antibody detecting Claspin in WB, IP and IHC (fp). Suitable for Human.

Quick Overview for Claspin antibody (AA 725-775) (ABIN7454603)

Target: Claspin (CLSPN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claspin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Claspin Antibody

Binding Specificity: AA 725-775

Purpose: Rabbit anti-Claspin Antibody, Affinity Purified

Predicted Reactivity: Orangutan,Gorilla,Chimpanzee

Purification: Affinity Purified

Immunogen: between AA 725 and 775

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 1 - 5 μg/mg lysate

WB: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Claspin

Target: Claspin (CLSPN)

Alternative Name: Claspin

Background: Background: Claspin is a checkpoint protein that interacts with and regulates checkpoint kinase 1 (Chk1) in response to replication stress. The interaction between claspin and Chk1 has been demonstrated to be required for ATR-dependent Chk1 activation. The interaction involves the phosphorylation of claspin that may be accomplished by Chk1 kinase activity. Claspin has been found to localize to chromatin in both the presence and absence of damaged DNA and to associate with PCNA, as an essential component of the DNA replication machinery. This association with the replication machinery may indicate a role for claspin in linking replication to DNA damage signaling and Chk1 activation.

Gene ID: 63967

UniProt: Q9HAW4