NAMPT antibody (AA 400-450)

Catalog No. ABIN7454619

This Rabbit Polyclonal antibody specifically detects NAMPT in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for NAMPT antibody (AA 400-450) (ABIN7454619)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-NAMPT Antibody

Binding Specificity: AA 400-450

Purpose: Rabbit anti-PBEF Antibody, Affinity Purified

Predicted Reactivity: Rat,Pig

Purification: Affinity Purified

Immunogen: between AA 400 and 450

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF

Background: Background: PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.

Gene ID: 10135

NCBI Accession: NP_005737

UniProt: P43490