BUB1 antibody (AA 1-50)

Catalog No. ABIN7454620

The Rabbit Polyclonal anti-BUB1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect BUB1 in samples from Human.

Quick Overview for BUB1 antibody (AA 1-50) (ABIN7454620)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BUB1 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-Bub1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: Bub1

Background: Background: Bub1 is cell cycle checkpoint kinase and component of the spindle checkpoint machinery. Bub1 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub1 functions to target Bub1-related protein (BubR1), Mad2, and polo-like kinase to kinetochores. It also functions to phosphorylate Cdc20 and inhibit ubiquitination of Pds1/securin by the anaphase-promoting complex (APC).

Gene ID: 699

NCBI Accession: NP_004327

UniProt: O43683