RNF20 antibody (AA 125-175)

Catalog No. ABIN7454669

This anti-RNF20 antibody is a Rabbit Polyclonal antibody detecting RNF20 in WB, IP and IHC (fp). Suitable for Human and Mouse.

Quick Overview for RNF20 antibody (AA 125-175) (ABIN7454669)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF20 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RNF20 Antibody

Binding Specificity: AA 125-175

Purpose: Rabbit anti-RNF20 Antibody, Affinity Purified

Predicted Reactivity: Chicken,Bovine

Purification: Affinity Purified

Immunogen: between AA 125 and 175

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: RNF20

Background: Background: The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription.

Gene ID: 56254

NCBI Accession: NP_062538

UniProt: Q5VTR2