RAD9A antibody (C-Term)

Catalog No. ABIN7454682

This Rabbit Polyclonal antibody specifically detects RAD9A in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for RAD9A antibody (C-Term) (ABIN7454682)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RAD9A Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Rad9 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 350 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: Rad9

Background: Background: Rad9 is a cell-cycle checkpoint protein with 3'-5' exonuclease activity and is part of the 9-1-1 complex. The 9-1-1 complex, also called the checkpoint clamp, is a trimeric complex that includes Rad9, Hus1, and Rad1. This complex is recruited by the RAD17/RFC clamp loader to DNA lesions where it aids in the activation of Chk2 by ATM and ATRIP and the subsequent triggering of the checkpoint signaling cascade. In addition to its role in sensing DNA damage, Rad9 also functions in the base excision repair process of DNA repair.

Gene ID: 5883

NCBI Accession: NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus