TOP2B antibody (C-Term)

Catalog No. ABIN7454697

This Rabbit Polyclonal antibody specifically detects TOP2B in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for TOP2B antibody (C-Term) (ABIN7454697)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TOP2B Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Topo II Beta Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1570 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 to 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: Topo II Beta

Background: Background: Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II beta is one of two described isoforms that are likely products of a gene duplication event. TopoII beta is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.

Gene ID: 7155

NCBI Accession: NP_001059

UniProt: Q02880