CHD3 antibody (AA 1575-1625)

Catalog No. ABIN7454727

This Rabbit Polyclonal antibody specifically detects CHD3 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CHD3 antibody (AA 1575-1625) (ABIN7454727)

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CHD3 Antibody

Binding Specificity: AA 1575-1625

Purpose: Rabbit anti-CHD3 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1575 and 1625

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CHD3

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Alternative Name: CHD3

Background: Background: CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.

Gene ID: 1107

NCBI Accession: NP_001005273

UniProt: Q12873