MAP4K4 antibody (AA 550-600)

Catalog No. ABIN7454756

This anti-MAP4K4 antibody is a Rabbit Polyclonal antibody detecting MAP4K4 in WB, IP and IHC (fp). Suitable for Human.

Quick Overview for MAP4K4 antibody (AA 550-600) (ABIN7454756)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MAP4K4 Antibody

Binding Specificity: AA 550-600

Purpose: Rabbit anti-MAP4K4/HGK Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 550 and 600

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4/HGK

Background: Background: MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.

Gene ID: 9448

UniProt: O95819