EIF3S1 antibody (AA 208-258)

Catalog No. ABIN7454779

The Rabbit Polyclonal anti-EIF3S1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect EIF3S1 in samples from Human and Mouse.

Quick Overview for EIF3S1 antibody (AA 208-258) (ABIN7454779)

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EIF3S1 Antibody

Binding Specificity: AA 208-258

Purpose: Rabbit anti-eIF3J/EIF3S1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Orangutan

Purification: Affinity Purified

Immunogen: between AA 208 and 258

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EIF3S1

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Alternative Name: eIF3J/EIF3S1

Background: Background: Eukaryotic initiation factor 3 subunit J (eIF3J) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3J is proposed to act as a factor that further stabilizes the interaction between eIF3 and the ribosome.

Gene ID: 8669

NCBI Accession: NP_003749

UniProt: O75822

Pathways: Ribonucleoprotein Complex Subunit Organization