NUP50 antibody (AA 225-275)

Catalog No. ABIN7454784

This Rabbit Polyclonal antibody specifically detects NUP50 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for NUP50 antibody (AA 225-275) (ABIN7454784)

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-NUP50 Antibody

Binding Specificity: AA 225-275

Purpose: Rabbit anti-NUP50 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 225 and 275

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50

Background: Background: The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Nup50 (also called Npap60) appears to not be a fixed structural component of the NPC. Nup50 has been shown to be a component of the importin complex, a protein carrier complex that imports cargo from the cytoplasm to the nucleus through the NPC. In this complex, Nup50 appears to have importance in the late stages of import and functions in complex disassembly and importin recycling.

Gene ID: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation