KDM5B antibody (AA 1494-1544)

Catalog No. ABIN7454788

The Rabbit Polyclonal anti-KDM5B antibody has been validated for WB, IP and IHC (fp). It is suitable to detect KDM5B in samples from Human.

Quick Overview for KDM5B antibody (AA 1494-1544) (ABIN7454788)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KDM5B Antibody

Binding Specificity: AA 1494-1544

Purpose: Rabbit anti-JARID1B Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1494 and 1544

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: JARID1B

Background: Background: JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer.

Gene ID: 10765

NCBI Accession: NP_006609

UniProt: Q9UGL1

Pathways: Warburg Effect