EEA1 antibody (AA 100-150)

Catalog No. ABIN7454791

The Rabbit Polyclonal anti-EEA1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect EEA1 in samples from Human.

Quick Overview for EEA1 antibody (AA 100-150) (ABIN7454791)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EEA1 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit anti-EEA1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 100 and 150

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background: Background: Early endosomal autoantigen 1 (EEA1) bears an FYVE (Fab1p-YOPB-Vps27p-EEA1) domain which is an approximately 80 amino acid double-zinc binding motif that binds to PtdIns(3)P (phosphatidylinositol 3-phosphate). The FYVE domain is found in proteins involved in intracellular signaling, membrane trafficking, and cytoskeletal rearrangement. EEA1 binds to early endosomal membranes that contain PtdIns(3)P and plays a role in the docking and fusion of early endosomes.

Gene ID: 8411

NCBI Accession: NP_003557

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades