COBRA1 antibody (AA 530-580)

Catalog No. ABIN7454796

This Rabbit Polyclonal antibody specifically detects COBRA1 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for COBRA1 antibody (AA 530-580) (ABIN7454796)

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COBRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-COBRA1 Antibody

Binding Specificity: AA 530-580

Purpose: Rabbit anti-COBRA1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 530 and 580

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: COBRA1

Background: Background: COBRA1 (Cofactor of BRCA1) is also known as NELFB (negative elongation factor E). NELFB is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. COBRA1 was originally identified in a yeast two-hybrid screen of a human ovary cDNA library using the BRCA COOH terminus repeat, BRCT1, as the bait protein. In this study, COBRA1 was found to be involved in BRCT1-mediated chromatin remodeling. COBRA1 has also been found to interact with several nuclear receptors such as ERalpha and androgen receptor to modulate their transcriptional activity.

Gene ID: 25920

NCBI Accession: NP_056271

UniProt: Q8WX92