MED13L antibody (AA 750-800)

Catalog No. ABIN7454829

This Rabbit Polyclonal antibody specifically detects MED13L in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for MED13L antibody (AA 750-800) (ABIN7454829)

Target: MED13L (Mediator Complex Subunit 13-Like (MED13L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED13L antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MED13L Antibody

Binding Specificity: AA 750-800

Purpose: Rabbit anti-MED13L Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 750 and 800

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MED13L

Target: MED13L (Mediator Complex Subunit 13-Like (MED13L))

Alternative Name: MED13L

Background: Background: Mediator complex subunit 13-like is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. [taken from the Universal Protein Resource (UniProt) Q71F56].

Gene ID: 23389

NCBI Accession: NP_056150

UniProt: Q71F56

Pathways: Regulation of Lipid Metabolism by PPARalpha