Vinculin antibody (AA 1016-1066)

Catalog No. ABIN7454836

The Rabbit Polyclonal anti-Vinculin antibody has been validated for WB, IP and IHC (fp). It is suitable to detect Vinculin in samples from Human and Mouse.

Quick Overview for Vinculin antibody (AA 1016-1066) (ABIN7454836)

Target: Vinculin (VCL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Vinculin Antibody

Binding Specificity: AA 1016-1066

Purpose: Rabbit anti-Vinculin Antibody, Affinity Purified

Predicted Reactivity: Rat,Chicken,Pig

Purification: Affinity Purified

Immunogen: between AA 1016 and 1066

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 15 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in vinculin are the cause of cardiomyopathy dilated type 1W, a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia [taken from NCBI Entrez Gene (GeneID: 7414)].

Gene ID: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2