EYA2 antibody (AA 100-150)

Catalog No. ABIN7454851

The Rabbit Polyclonal anti-EYA2 antibody is suitable to detect EYA2 in samples from Human. It has been validated for WB, IP and IHC (fp).

Quick Overview for EYA2 antibody (AA 100-150) (ABIN7454851)

Target: EYA2 (Eyes Absent Homolog 2 (Drosophila) (EYA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EYA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EYA2 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit anti-EYA2 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 100 and 150

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EYA2

Target: EYA2 (Eyes Absent Homolog 2 (Drosophila) (EYA2))

Alternative Name: EYA2

Background: Background: EYA2 (eyes absent homolog 2) functions as a tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX. EYA2 promotes efficient DNA repair by dephosphorylating H2AX and promoting the recruitment of DNA repair complexes containing MDC1. EYA2 coactivates SIX1. Together with SIX1 and DACH2 EYA2 seems to be involved in myogenesis. EYA2 may be involved in development of the eye [taken from the Universal Protein Resource (UniProt) O00167].

Gene ID: 2139

NCBI Accession: NP_005235

UniProt: O00167