U2AF2 antibody (AA 430-475)

Catalog No. ABIN7454940

This Rabbit Polyclonal antibody specifically detects U2AF2 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for U2AF2 antibody (AA 430-475) (ABIN7454940)

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-U2AF2 Antibody

Binding Specificity: AA 430-475

Purpose: Rabbit anti-U2AF65 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: Between AA 430 and 475

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF65

Background: Background: U2 auxiliary factor (U2AF) is comprised of a large and a small subunit and is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. Splicing factor U2 auxiliary factor 65 kDa subunit (U2AF65) is the large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. U2AF65 binds to the polypyrimidine tract of introns early during spliceosome assembly [taken from NCBI Entrez Gene (Gene ID: 11338)].

Gene ID: 11338

NCBI Accession: NP_009210

UniProt: P26368