POLR2C antibody (AA 225-275)

Catalog No. ABIN7454959

The Rabbit Polyclonal anti-POLR2C antibody is suitable to detect POLR2C in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for POLR2C antibody (AA 225-275) (ABIN7454959)

Target: POLR2C (Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa (POLR2C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-POLR2C Antibody

Binding Specificity: AA 225-275

Purpose: Rabbit anti-RPB3 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: Between AA 225 and 275

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for POLR2C

Target: POLR2C (Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa (POLR2C))

Alternative Name: RPB3

Background: Background: RPB3 (RNA polymerase II subunit 3) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft [taken from the Universal Protein Resource (UniProt) P19387].

Gene ID: 5432

NCBI Accession: NP_116558

UniProt: P19387

Pathways: Regulatory RNA Pathways