XPO5 antibody (AA 1154-1204)

Catalog No. ABIN7454998

The Rabbit Polyclonal anti-XPO5 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect XPO5 in samples from Human and Mouse.

Quick Overview for XPO5 antibody (AA 1154-1204) (ABIN7454998)

Target: XPO5 (Exportin 5 (XPO5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPO5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-XPO5 Antibody

Binding Specificity: AA 1154-1204

Purpose: Rabbit anti-Exportin-5 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 1154 and 1204

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for XPO5

Target: XPO5 (Exportin 5 (XPO5))

Alternative Name: Exportin-5

Background: Background: Exportin-5 mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). Exportin-5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. Exportin-5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport [taken from the Universal Protein Resource (UniProt) Q9HAV4].

Gene ID: 57510

NCBI Accession: NP_065801

UniProt: Q9HAV4

Pathways: Regulatory RNA Pathways, Protein targeting to Nucleus