CD43 antibody (AA 350-400)

Catalog No. ABIN7455030

This Rabbit Polyclonal antibody specifically detects CD43 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CD43 antibody (AA 350-400) (ABIN7455030)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CD43 Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-CD43 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: Background: CD43 is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, CD43 is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune respons [taken from NCBI Entrez Gene (Gene ID: 6693)].

Gene ID: 6693

NCBI Accession: NP_001025459

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity