Recombinant MED1 antibody (AA 1550-1581)

Catalog No. ABIN7455140

This anti-MED1 antibody is a Rabbit Monoclonal antibody detecting MED1 in WB, IP, ICC, IHC (fp) and FACS. Suitable for Human and Mouse.

Quick Overview for Recombinant MED1 antibody (AA 1550-1581) (ABIN7455140)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS)

Clone: BLR037F

Product Details anti-MED1 Antibody

Binding Specificity: AA 1550-1581

Purpose: Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]

Predicted Reactivity: Orangutan

Immunogen: between AA 1550 and 1581 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1

Background: Background: MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) Q15648].

Gene ID: 5469

NCBI Accession: NP_004765

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha