Recombinant T-Bet antibody (AA 485-535)

Catalog No. ABIN7455151

The Rabbit Monoclonal anti-T-Bet antibody has been validated for WB, IP, ICC, FACS and IHC (fp). It is suitable to detect T-Bet in samples from Human.

Quick Overview for Recombinant T-Bet antibody (AA 485-535) (ABIN7455151)

Target: T-Bet

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR110H

Product Details anti-T-Bet Antibody

Binding Specificity: AA 485-535

Purpose: Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]

Immunogen: between AA 485 and 535 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: T-bet/TBX21

Background: Background: T-bet/TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX21 is a lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and activating transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. [taken from NCBI Entrez Gene (Gene ID: 30009)].

Gene ID: 30009

NCBI Accession: NP_037483

UniProt: Q9UL17