T-Bet antibody (Middle Region)

Catalog No. ABIN2779750

The Rabbit Polyclonal anti-T-Bet antibody is suitable to detect T-Bet in samples from Human, Mouse, Rat, Cow, Dog, Pig, Rabbit and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for T-Bet antibody (Middle Region) (ABIN2779750)

Target: T-Bet

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-T-Bet Antibody

Binding Specificity: Middle Region

Purpose: TBX21 Antibody - middle region

Sequence: SIPSPPGPNC QFLGGDHYSP LLPNQYPVPS RFYPDLPGQA KDVVPQAYWL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against TBX21. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TBX21

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32612-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background:

Background Information: TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. It is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Gene Name: T-box 21

Alternative Symbols: TBET, T-PET, T-bet, TBLYM

Protein Name: T-box transcription factor TBX21

Molecular Weight: 58 kDa

Gene ID: 30009

NCBI Accession: NP_037483

UniProt: Q9UL17