Recombinant Cadherin 5 antibody (AA 48-599)

Catalog No. ABIN7455190

The Rabbit Monoclonal anti-Cadherin 5 antibody has been validated for WB, IP, ICC, IF (p) and IHC (fp). It is suitable to detect Cadherin 5 in samples from Human.

Quick Overview for Recombinant Cadherin 5 antibody (AA 48-599) (ABIN7455190)

Target: Cadherin 5 (CDH5)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR091G

Product Details anti-Cadherin 5 Antibody

Binding Specificity: AA 48-599

Purpose: Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR091G]

Immunogen: residues 48-599 (ECD)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: VE-Cadherin

Background: Background: Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in adjacent cells. VE-Cadherin, or Cadherin-5 plays an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton and acts in concert with KRIT1 and MPP5 to establish and maintain correct endothelial cell polarity and vascular lumen. [taken from the Universal Protein Resource (UniProt) P33151].

Gene ID: 1003

NCBI Accession: NP_001786

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2