Recombinant SMC1A antibody (C-Term)

Catalog No. ABIN7455193

The Rabbit Monoclonal anti-SMC1A antibody has been validated for WB, FACS, IF (p), IP, ICC and IHC (fp). It is suitable to detect SMC1A in samples from Human and Mouse.

Quick Overview for Recombinant SMC1A antibody (C-Term) (ABIN7455193)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BL-205-2G8

Product Details anti-SMC1A Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8]

Predicted Reactivity: Rat,Bovine

Immunogen: between AA 1200 to 1233 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/mg lysate

ICC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: Background: Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.

Gene ID: 8243

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance