Recombinant CD276 antibody (C-Term)

Catalog No. ABIN7455195

This Rabbit Monoclonal antibody specifically detects CD276 in WB, FACS, IP, ICC, IF (p) and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant CD276 antibody (C-Term) (ABIN7455195)

Target: CD276

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD276 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BLR025F

Product Details anti-CD276 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-B7-H3/CD276 Recombinant Monoclonal Antibody [BLR025F]

Immunogen: between AA 500 to 534 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/mg lysate

ICC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CD276

Target: CD276

Alternative Name: B7-H3/CD276

Background: Background: CD276 may participate in the regulation of T-cell-mediated immune response. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It may also be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces and could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy [taken from the Universal Protein Resource (UniProt) Q5ZPR3].

Gene ID: 80381

NCBI Accession: NP_001019907

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints